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Flow injection immunoassay for insulin using a new synergistic enhanced chemiluminescence / 中华检验医学杂志
Chinese Journal of Laboratory Medicine ; (12)2001.
Artículo en Chino | WPRIM | ID: wpr-582135
ABSTRACT
Objective To establish a new non radioimmunoassay for detecting insulin. Methods We put together the techniques of chemiluminescent analysis, flow injection analysis and immunoassay and established a method,flow injection chemiluminescent immunoassay, for insulin determination using sandwhich procedure with the horseradish peroxdase (HRP) as label of antibody and using the detection system of synergistic enhanced chemiluminescence of para phenylpenol and sodium tetraphenylborate with HRP catalysed oxidation of luminol. Results Satisfactory results were obtained in the dynamic range 0.15~60.00 ?U with a relative coefficient of 0 997 2 ( P

Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Idioma: Chino Revista: Chinese Journal of Laboratory Medicine Año: 2001 Tipo del documento: Artículo

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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Idioma: Chino Revista: Chinese Journal of Laboratory Medicine Año: 2001 Tipo del documento: Artículo