The role of dephosphorylated RB protein in human breast cancer cell apoptosis / 医学研究生学报
Journal of Medical Postgraduates
;
(12)2003.
Artículo
en Chino
| WPRIM
| ID: wpr-584341
ABSTRACT
Objective:
To investigate the role of the expression of dephosphorylated RB protein for cell apoptosis in human breast cancer.Methods:
In our works, human breast cell lines (MCF-7/S,the chemosensitive cell line and MCF-7/Adr, the chemoresistent cell line)were evaluated. The expressive levels of dephosphorylated RB protein were detected with immunocytochemistry. Apoptosis rates were determined by flow cytometry(FCM).Results:
Before treatment of ADR, phosphorylated RB protein was positive in two cell lines, but dephosphorylated RB protein was negative; After treatment of different concentration ADR, when the concentration of ADR was increased, expression of dephosphorylated RB protein elevated accordingly in MCF-7/S, but no significant change in MCF-7/Adr. Apoptosis and cycle was detected by FCM assays shows ADR induced apoptosis of MCF-7/S more than MCF-7/Adr(P
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Idioma:
Chino
Revista:
Journal of Medical Postgraduates
Año:
2003
Tipo del documento:
Artículo
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