Role of transforming growth factor?1 in pathogenesis of human polycystic kidney disease / 医学研究生学报

ABSTRACT

Objective:To investigate characteristic expression of TGF-?1 in human ADPKD and clarify its role in the development and progression of human ADPKD. Methods:Cyst fluid, urinary and plasma TGF-?1 levels were determined by ELISA in 39 ADPKD patients. The results were compared with those of normal subjects and of patients with simple renal cyst. TGF-?1, TGF-?1 receptors types Ⅰ, types Ⅱ, CTGF mRNA and proteins in the kidneys of human ADPKD were examined by in situ hybridization and immunohistochemistry. The relationship of the above fibrosing-associated indicators with the degree of interstitial fibrosis was analyzed. Results:Plasma TGF-?1 level was the highest among body fluids. In ADPKD and simple renal cyst, TGF-?1 levels were significantly higher than those in normal subjects (15.12 ?8.53)?g/L vs (5.41?1.31) ?g/L,P