Research on whole blood control materials for lymphocyte subset counting by flow cytometry / 中华检验医学杂志

ABSTRACT

Objective To research on a whole blood control material for lymphocyte subset counting by flow cytometry(FCM).Methods To detect lymphocyte subset in whole blood with different preservers by flow cytometric multi-color analysis.Results whole blood control material for lymphocyte subset counting by FCM was prepared.In 2-8℃ refrigerator, the light scatter and CD45 of leukocytes of whole blood control were stable in 72 days. The cluster of lymphocyte, monocyte, neutrophil in plot were separated easily from debris. The lymphocyte subset of whole blood control can be counted by FSC/SSC or CD45/SSC gating. The variation of lymphocyte subset count was less in different preserving day. The coefficient of variation (CV) of lymphocyte subset count was less than 6.5% in our laboratory and less than 13% in external quality assessment among 56 laboratories in China.Conclusion The whole blood control prepared by us can be used for internal quality control and external quality assessment in lymphocyte subset counting by FCM, it is very important signification to ensure the quality and accuracy of lymphocyte subset count.