The expression and activity of Caspase 3 in E.coli BL21 / 医学研究生学报
Journal of Medical Postgraduates
;
(12)2003.
Artículo
en Chino
| WPRIM
| ID: wpr-585559
ABSTRACT
Objective:
To study the expression and activity of the apoptosis protease-Caspase 3 in(E.coli) BL21(DE3).Methods:
The cDNA of Caspase 3 was amplified by PCR and inserted into the plasmid pCMV-Myc,it is then cloned to prokaryotic expression vector pGEX-6p,after which Caspase 3 was induced by IPTG.The protein induced was identified by SDS-PAGE and Western blot.Results:
After induced by IPTG for 3 hours,the concentration of Caspase 3 reached the highest level.Conclusion:
Active Caspase 3 can be induced within E.coli BL21(DE3),further research can be done about the role of Caspase 3 in apoptosis.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Idioma:
Chino
Revista:
Journal of Medical Postgraduates
Año:
2003
Tipo del documento:
Artículo
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