An Orthogonal Experiment in the Brefeldin a Fermentation of Pencillium / 微生物学通报

ABSTRACT

Through orthogonal experiment,the optimal conditions were given for producing extracellular BFA of Penicillium.Its optimal conditions were as follows(g/L) potato 200,glucose 20,(NH_(4))_(2)SO_(4) 4.0, KH_(2)PO_(4) 1.0,MgSO_(4)?7H_(2)O 2.0,CaCO_(3) 5.0,the pH was natural,culture medium volume 100mL/250mL,culture temperature 28℃,revolutions 120r/min and culture time 7 days.The biggest production of BFA was 151.6(mg/L.)