Quantitative Real-time Detection of Spontaneous Perforin(PFR) Gene Expression in Peripheral Blood Mononuclear Cell(PBMC) by Polymerase Chain Reaction / 中国运动医学杂志

ABSTRACT

Objective To develop a method for quantitative real-time detection of perforin(PFR) using polymerase chain reaction(PCR).Methods Gene expression of PFR in peripheral blood mononuclear cell(PBMC) from 25 healthy individuals was detected by PCR.An endogenous"housekeeping" gene GAPDH was used to normalize the results.Results and Conclusion The spontaneous PFR mRNA expression in PBMC in a given sample could be measured by PCR with relativity correlation coefficient of r=1.