Determination of sodium valprate in plasma by capillary zone electrophoresis / 中华检验医学杂志
Chinese Journal of Laboratory Medicine
;
(12)2001.
Artículo
en Chino
| WPRIM
| ID: wpr-585998
ABSTRACT
Objective To establish a rapid determination of sodium valprate in plasma by capillary zone electrophoresis.Method Plasma was acidized by 1 mol/L HCl, VPA was extracted into organic phase (n-Hexane), then re-extracted into aqueous phase (0.05 mmol/L NaOH solution including 15% Ethanol). Capillary clone was 75 ?m(id)?37 cm. Electrolyte consisted of 15 mmol/L sodium salicylate, 0.5 mmol/L CTAB and 15% Ethanol (pH 5.7).Separating voltage was 20 kV, detection wave was 214 nm, temperature was 20℃,injection time was 5 s by press in negative.Result The linear ranger of concentration for standard drug was between 25~200 ?g/ml (r=0.999),the limit of detection was 0.35 ?g/ml, the average recovery of VPA was 87.4%,the average inter-day and intar-day CV were less than 4% and 6%. Conclusion This method is simple, rapid, reliable and correct, for determination of VPA in plasma.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Idioma:
Chino
Revista:
Chinese Journal of Laboratory Medicine
Año:
2001
Tipo del documento:
Artículo
Similares
MEDLINE
...
LILACS
LIS