Survivin gene-targeted siRNA inducing apoptosis of human osteosarcoma cell line U-2OS / 中国肿瘤生物治疗杂志

ABSTRACT

Objective: To investigate the proliferation and apoptosis of human osteosarcoma cell line U-2OS after sur-vivin gene was knocked down by siRNA synthesized in vitro. Methods: U-2OS cells were divided into 3 groups Group A (blank control) , group B ( transfected with non-specific siRNA) and group C( transfected with survivin-specific siRNA). The morphological changes of U-2OS cells were observed with fluorescent microscope. The expression of survivin mRNA and protein were detected by reverse transcription polymerase chain reaction ( RT-PCR) and Western blotting. The anti-proliferative effects were assessed by MTT and the rate of apoptosis was examined by flow cytometry in 3 groups. The results of the 3 groups were analyzed and compared. Results: Survivin specific siRNA significantly down-regulated mRNA and protein expression of survivin. Expression of survivin protein in group C was significantly lower than those in group A and B( P