Experimental study of the nitric oxide-enhanced damaging effect of cyclophosphamide therapy on leukemia cells / 医学研究生学报

ABSTRACT

Objective:To observe whether nitric oxide (NO) could enhance the damaging effect of cyclophosphamide on L1210 cells cultured in vitro, and to investigate the mechanism of this action. Methods:L1210 cells were co-cultured with 3T3 cells in DMEM medium supplemented with cyclophosphamide (400 ?g/ml). The L1210 cells were divided into three groups based on different transfected 3T3 cells: pcDNA3.0-iNOS plasmid transfected 3T3 cells (Group 1), pcDNA3.0 plasmid transfected 3T3 cells (Group 2), pcDNA3.0-iNOS plasmid transfected 3T3 cells plus DEVD-CHO(Group 3). The viability and apoptosis rate of L1210 cells at different culture periods were determined by trypan blue exclusion and TUNNEL method, respectively. And the cell cycles at G_1 and S phase were detected by flow cytometry. Results:①After cyclophosphamide treatment, the viability of L1210 cells was significantly lower in Group 1 than that in Group 2 during 12-72 h (P