Effect of CpG-ODN on phenotypes,function of monayte-derived dendritic cells and expression of STAT and SOCS in patients with chronic hepatitis B / 基础医学与临床

ABSTRACT

Objective To study the effect of CpG containing oligodeoxynucleotide(CpG-ODN) on the phenotypes,function and expression of signal transductors and activators of transcription(STAT) and suppressors of cell signaling(SOCS) of peripheral blood dendritic cells(DC) in patients with chronic hepatitis B(CHB).Methods Monocytes isolated from peripheral blood of CHB and healthy volunteers were cultured and induced by granulocyte-monocyte colony stimulating factor(GM-CSF) and interleukin-4(IL-4).Then,CpG-ODN or TNF-? were added to stimulate the immatured DC,their effects on the phenotypes and function of DC were evaluated.(The expression of) signaling molecules of STAT1,3,4,5,6 and SOCS1,3 in DC were detected by Western blotting.Results The(expression) rates of HLA-DR of DC in CHB with the treatment of CpG-ODN or TNF-? were obviously increased;Both the IL-12p70 expression and stimulating capacity of DC to allogenic T lymphocytes were improved significantly in CpG-ODN group and TNF-? group(P