Response of astrocytes to sleep deprivation and sleep recovery and its relationship with neurons at rat's suprachiasmaticus nucleus / 医学研究生学报

ABSTRACT

Objective:To investigate the response of the astrocytes to sleep deprivation and sleep recovery at rat~(,)s SCN,as well as its relationship with neurons.Methods:12 Sprague-Damley rats were divided into four groups at random.Two groups were deprived of sleep by housing them on the small platforms over water for 24h and then one group recovered sleep for 3h.Controls were housed in the normal cage and on the large platforms over water.There were 3 rats in each group.The effects of sleep deprivation and sleep recovery on the expression of glial fibrillary acidic protein(GFAP) and Fos protein were investigated by immunohistochemistry.Results:The expressions of GFAP and Fos were increased obviously at SCN after sleep deprivation,where both of them were depressed after sleep recovery and the tendencies of these changes were almost at equal pace.Conclusion:It is possible that the astrocytes regulate sleep together with neurons.