Wound Infectivity by Vibrio parahaemolyticus Derived from Seacoast / 中华医院感染学杂志

ABSTRACT

OBJECTIVE To study the infectivity of the Vibrio parahaemolyticus isolated from the brine. METHODS Virulence test group to divide 35 Kunbai mice into 4 subgroups at random to inject V. parahaemolyticus into the mice′ abdominal cavity of the test group, Staphylococcus aureus or Escherichia coli into those of the positive control groups and aseptic physiological saline into those of negative control group. Wound infection groupto divide 35 SPF mice into 4 subgroups at random after the legs injured test subgroup (soaked in artificial brine with bacteria ), two positive control subgroups(with S.aureus or E.coli), negative control subgroup(soaked in aseptic physiological saline). To observe the general condition, blood routine, hemoculture, viscera culture of the mice, after 4 days the mice were sacrificed and examined the viscera with pathological analysis. RESULTS Virulence test group the hemoculture of one mouse was positive after injected the bacteria into its abdominal cavity for 12 hours, and viscera bacterial culture was positive. Wound infection test groupthe ratio of wound infection was 100%,the positive ratio of both the hemoculture and the viscera bacteria culture were 10% after the wound soaked in bacteria solution. There were a great deal of neutrophilic granulocytes infiltration and cellulitis in the striated muscles of wound limbs through pathological examination. The infection of severe degree corresponded with the positive control groups, there was no inflammatory reaction in negative control group. CONCLUSIONS The V. parahaemolyticus isolated from the brine has infectivity and makes the wound of the mice be infected and hematoseptic when the concentration reached 10~6 CFU/ml .