Drug Resistance Genes of Eight CTX-M-14 Variant Genotypes from Klebsiella pneumoniae and Escherichia coli / 中华医院感染学杂志

ABSTRACT

OBJECTIVE To analyze the sequences of drug resistance genes of the novel CTX-M type of extended spectrum ?-lactamases((ESBLs)) in Hefei Anhui Province. METHODS A pair of primers were designed to detect the groups of CTX-M-9 by PCR from 98 strains confirmed(ESBLs)-producing Klebsiella pneumoniae and Escherichia coli isolated between 1999 and 2000 in Hefei,and then sequenced the productions of PCR,Compared the sequences on blast via GenBank and analyzed the molecular structure to find out whether the change in nucleotides had induced the destitution of amino acid in specific site. RESULTS Of 29 strains,8 variant sequences of CTX-M-14 had been discovered in several sites and resulted in the destitution of amino acid. CONCLUSIONS There are novel CTX-M types of(ESBLs) in Hefei,which come from CTX-M-14.