Piezoelectric Gene Biosensors for Detection of Pseudomonas aeruginosa Using Signal Amplification Method with Cationic Conjugated Polymers / 中华医院感染学杂志
Chinese Journal of Nosocomiology
;
(24)2005.
Artículo
en Chino
| WPRIM
| ID: wpr-587764
ABSTRACT
OBJECTIVE To discuss the feasibility of signal amplification method with cationic conjugated polymers(liposome) applied during the detection of Pseudomonas aeruginosa using piezoelectric gene biosensors.(METHODS) Oligonucleotide probe for P.aeruginosa was immobilized on the surface of gene sensor array and(hybridized) by PCR production of P.aeruginosa.After hybridization,liposome was added.The frequency shifts were recorded and compared with those ones of the control groups.RESULTS The frequency shifts were(significantly) increasing when adding liposome and the compatible concentration of liposome was 0.8?g/?l.(CONCLUSIONS) Liposome signal amplification is proved to be an effective method to amplify the piezoelectric(signal).
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Tipo de estudio:
Estudio diagnóstico
Idioma:
Chino
Revista:
Chinese Journal of Nosocomiology
Año:
2005
Tipo del documento:
Artículo
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