Purification of AmpC beta-lactamase in Enterobacter cloacae / 临床检验杂志
Chinese Journal of Clinical Laboratory Science
;
(12)2006.
Artículo
en Chino
| WPRIM
| ID: wpr-588171
ABSTRACT
Objective To purify AmpC beta-lactamase from Enterobacter cloacae and study its physical and chemical characteristics.Methods The bacteria were fragmentized by ultrasonication and AmpC beta-lactamase was purified by ion-exchange.The properties of the purified enzyme,such as molecular weight and isoelectric point were determined by electrophoresis.Results The purified AmpC beta-lactamase was obtained and its specificity to substrate was sufficient for classification of class C cephalosporinase.Conclusion AmpC can be successfully purified from Enterobacter cloacae and is effective for application.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Idioma:
Chino
Revista:
Chinese Journal of Clinical Laboratory Science
Año:
2006
Tipo del documento:
Artículo
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