Expression of ion channel gene during MSCs differentiating into cardiomyocytes / 基础医学与临床

ABSTRACT

Objective To study the expression of L-type calcium channel gene(?1c) and transient outward potassium channel gene(kv4.3)during the procession of MSCs differentiating into cardiomyocytes.Methods MSCs were isolated cultured,passaged,purified,and then exposed to 5-aza for 24 hours.The expression of ?1C and kv4.3 gene was detected by reverse transcription-polymerase chain reaction.Result MSCs had already expressed ?1c and kv4.3 mRNA before 5-aza treatment.The expression of kv4.3 was significantly increased after 5-aza exposure at 24 hoursand at 4th,7th day and 14th day,wheras the expression of ?1C was impaired.Conlusion L-type calcium channel and transient outward potassium channel played important roles during procession of MSCs differentiating into cardiomyocytes.