Inhibitory effect of sodium phenylbutyrate combined with gefitinib on PKC of NB4 cells / 吉林大学学报(医学版)

ABSTRACT

Objective To investigate the inhibitory effect of sodium phenylbutyrate(SPB) combined with gefitinib on NB4 cells.Methods The NB4 cells were divided into 8 groups randomlycontrol group,PMA group,H-7 group,SPB+PMA group,gefitinib group,gefitinib+PMA group,SPB+gefitinib group.The inhibitory rate of medicine was detected by MTT,the apoptosis of NB4 cells was detected by flow cytometry,the expressions of PKC,CDK,and P53 were detected with Western blotting method.Results The NB4 cells were suppressed obviously.The rate of growth inhibition and proliferation index in SPB+gefitinib group were 92.8% and 5.96%,those in SPB group were 45.1% and 29.6%,and gefitinib group were 61.0% and 35.9%,the differences were significant(P