Cytoprotective effect of rhamnetin on miconazole-induced H9c2 cell damage
Nutrition Research and Practice
;
: 586-591, 2015.
Artículo
en Inglés
| WPRIM
| ID: wpr-59001
ABSTRACT
BACKGROUND/OBJECTIVES:
Reactive oxygen species (ROS) formation is closely related to miconazole-induced heart dysfunction. Although rhamnetin has antioxidant effects, it remained unknown whether it can protect against miconazole-induced cardiomyocyte apoptosis. Thus, we investigated the effects of rhamnetin on miconazole-stimulated H9c2 cell apoptosis. MATERIALS/METHODS:
Cell morphology was observed by inverted microscope and cell viability was determined using a WelCount(TM) cell proliferation assay kit. Miconazole-induced ROS production was evaluated by fluorescence-activated cell sorting with 6-carboxy-2',7'-dichlorofluoroscein diacetate (H2DCF-DA) stain. Immunoblot analysis was used to determine apurinic/apyrimidinic endonuclease 1 (APE/Ref-1) and cleaved cysteine-aspartic protease (caspase) 3 expression. NADPH oxidase levels were measured using real-time polymerase chain reaction.RESULTS:
Miconazole (3 and 10 microM) induced abnormal morphological changes and cell death in H9c2 cells. Rhamnetin enhanced the viability of miconazole (3 microM)-treated cells in a dose-dependent manner. Rhamnetin (1 and 3 microM) treatment downregulated cleaved caspase 3 and upregulated APE/Ref-1 expression in miconazole-stimulated cells. Additionally, rhamnetin significantly reduced ROS generation.CONCLUSIONS:
Our data suggest that rhamnetin may have cytoprotective effects in miconazole-stimulated H9c2 cardiomyocytes via ROS inhibition. This effect most likely occurs through the upregulation of APE/Ref-1 and attenuation of hydrogen peroxide levels.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Regulación hacia Arriba
/
Supervivencia Celular
/
Muerte Celular
/
Especies Reactivas de Oxígeno
/
Apoptosis
/
NADPH Oxidasas
/
Miocitos Cardíacos
/
Proliferación Celular
/
Caspasa 3
/
Reacción en Cadena en Tiempo Real de la Polimerasa
Idioma:
Inglés
Revista:
Nutrition Research and Practice
Año:
2015
Tipo del documento:
Artículo
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