A Novel Specific Small Molecule Peptide for Non-small Cell Lung Cancer Cell A549 / 生物化学与生物物理进展

ABSTRACT

To screen small molecule peptide specific binding to non-small cell lung cancer cell (A549) using the "one-bead one-peptide" combinatorial library. Twenty-nine positive beads binding to A549 cell were totally obtained after primary screening. Consensus peptide sequence of -NGXG-was identified by amino acid sequencing in ten beads. Peptide cNGQGEQc was re-synthesized on beads and further studied for its cell specificity, alanine scanning and site-directed deletion. The results showed that cNGQGEQc is specific for cell attachment to non-small cell lung cancer cells including A549, Calu-1 and H178, but not to other tumor cell lines. Both motif of -NGXG-and the length of six peptides are very important for A549 adhesion. Peptide cNGQGEQc labeled with FITC can specifically bind to A549 cell. In a blocking assay with anti-integrin antibodies(?1～6, ?v/?1～5), cell adhesion of A549 to peptide beads was obviously inhibited by integrin ?3 combining with any ? subunits. Results suggested that small molecule peptide cNGQGEQc can bind specifically to non-small cell lung cancer cell A549 via integrin ?3 on cell surface.