Development of enzyme-linked immunosorbent assay method for anti-B lymphocyte stimulator autoantibodies and its preliminary clinical application / 医学研究生学报
Journal of Medical Postgraduates
;
(12)2003.
Artículo
en Chino
| WPRIM
| ID: wpr-590134
ABSTRACT
Objective:
To investigate the prevalence of the antibodies against the B lymphocyte stimulator(BlyS),(BlyS-Ab) in patients with clinical diseases,and to explore the role of BlyS-Ab in the pathogenesis of autoimmune diseases(AID).Methods:
By optimizing experimental conditions,we developed ELISA methods for serum levels of BlyS-Ab,followed by methodological and clinical application assessment and investigation of the relationship of serum BlyS-Ab levels with the titers of anti-nucleus antibodies(ANA) and other autoantibodies.Results:
The intra-assay precision,namely the average coefficient values of BlyS-Ab was 7.8%,and the specific inhibition rate for BlyS-Ab by series concentration of BlyS was up to 77.3%.Clinical investigation revealed that the serum levels of BlyS-Ab was significantly higher in patients with autoimmune diseases(including SLE,RA,myasthenia gravis) and other medical cases(such as allergic purpurics,chronic nephritis,etc.) than in the normal control(P
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Idioma:
Chino
Revista:
Journal of Medical Postgraduates
Año:
2003
Tipo del documento:
Artículo
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