Effect of ATP sensitive potassium channel opener on neuronal apoptosis and the expression of caspase-8 in rats following cerebral ischemia-reperfusion / 临床神经病学杂志

ABSTRACT

Objective To study the effect of ATP sensitive potassium channel(KATP)opener on neuronal apoptosis and the expression of caspase-8 mRNA and protein in rats following cerebral ischemia-reperfusion.Methods 200 male Wistar rats were randomly divided into sham-operated group,control group,KATP opener precondition group(KATP opener group)and KATP opener and blocker precondition group(KATP blocker group).The middle cerebral artery ischemia-reperfusion models were established by using the intraluminal suture occlusion method.Neuronal apoptosis were detected by TUNEL staining,the expression of caspase-8 mRNA and protein were detected by immunohistochemical staining and RT-PCR methods.Results(1)The numbers of apoptotic cells in KATP opener group at 12 h,24 h,48 h,72 h after cerebral ischemia-reperfusion were significantly less than those in control group and KATP blocker group(P