Repeated Microneedle Stimulation Induces Enhanced Hair Growth in a Murine Model
Annals of Dermatology
;
: 586-592, 2016.
Artículo
en Inglés
| WPRIM
| ID: wpr-59030
ABSTRACT
BACKGROUND:
Microneedle is a method that creates transdermal microchannels across the stratum corneum barrier layer of skin. No previous study showed a therapeutic effect of microneedle itself on hair growth by wounding.OBJECTIVE:
The aim of this study is to investigate the effect of repeated microwound formed by microneedle on hair growth and hair growth-related genes in a murine model.METHODS:
A disk microneedle roller was applied to each group of mice five times a week for three weeks. First, to identify the optimal length and cycle, microneedles of lengths of 0.15 mm, 0.25 mm, 0.5 mm, and 1 mm and cycles of 3, 6, 10, and 13 cycles were applied. Second, the effect of hair growth and hair-growth-related genes such as Wnt3a, β-catenin, vascular endothelial growth factor (VEGF), and Wnt10b was observed using optimized microneedle. Outcomes were observed using visual inspection, real-time polymerase chain reaction, and immunohistochemistry.RESULTS:
We found that the optimal length and cycle of microneedle treatment on hair growth was 0.25 mm/10 cycles and 0.5 mm/10 cycles. Repeated microneedle stimulation promoted hair growth, and it also induced the enhanced expression of Wnt3a, β-catenin, VEGF, and Wnt10b.CONCLUSION:
Our study provides evidence that microneedle stimulation can induce hair growth via activation of the Wnt/β-catenin pathway and VEGF. Combined with the drug delivery effect, we believe that microneedle stimulation could lead to new approaches for alopecia.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Piel
/
Heridas y Lesiones
/
Inmunohistoquímica
/
Folículo Piloso
/
Factor A de Crecimiento Endotelial Vascular
/
Alopecia
/
Reacción en Cadena en Tiempo Real de la Polimerasa
/
Cabello
/
Métodos
Tipo de estudio:
Estudio pronóstico
Límite:
Animales
Idioma:
Inglés
Revista:
Annals of Dermatology
Año:
2016
Tipo del documento:
Artículo
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