Isolation and antioxidation of bioactive proteins from Meretrix meretrix / 中国海洋药物

ABSTRACT

Objective To investigate the antioxidation of bioactive proteins from the meat of Meretrix meretrix.Methods Three protein peaks(P1,P2 and P3) were isolated from the meat of Meretrix meretrix by homogenating,step precipitating with ammonium sulfate,chromatography with Sephadex G-150.CAT activity and SOD activity of these proteins were determined.Inhibition of the proteins to lipid peroxidation was determined.Results CAT activity of P2 was the highest,its specific activity was 77.0 U?mg-1.SOD activity of P3 was the highest,its specific activity was 68.8 U?mg-1.Inhibition of P3to lipid peroxidation was the most obvious,its inhibitive rate was 50% when the protein content was 20 ?g.SDS-PAGE results showed that the main composition of P1 was the protein,the Mr of which was 18.0 kD.The protein band of P2 corresponded with the protein band of contrast CAT,its Mr was 28.0 kD.The protein band of P3 corresponded with the protein band of contrast SOD,its relative Mr was 16.0 kD.Conclusion The bioactive proteins were isolated from the meat of Meretrix meretrix,and the bioactive proteins had antioxidation.