Repair of intestinal irradiation injury by Flk-1~+ MSCs and SP cells / 基础医学与临床

ABSTRACT

Objective Comparing biological characteristics and the roles in repairing of intestinal irradiation injury of bone marrow derived Flk-1+ MSCs and small intestinal derived SP cells.Methods Phenotypes of the two cell population were determined by flow cytometery.Flk-1+ MSCs and intestinal SP cells were infused into irradiated mice,distribution and differentiation of these cells were determined.Histopathological change and fibrosis were evaluated.Results The phenotypes of Flk-1+ MSCs and intestinal SP cells were quite different.Both Flk-1+ MSCs and intestinal SP cells could home to injury tissue rapidly,participate intestinal epithelial repair,and alleviate fibrosis and collagen deposition after irradiation.SP cells mostly engrafted into intestinal epithelium,while Flk-1+ MSCs partly engrafted into interstitial region as well as epithelium.Conclusion Flk-1+ MSCs and intestinal SP cells may alleviate symptoms effectively,though their mechanisms are disparite.