Effect of Sox-9 on degeneration of lumbar intervertebral disc

Jiyou LI

ABSTRACT

BACKGROUND:

It is possible to change the normal structure of lumbar intervertebral disc,even worse,to accelerate the degeneration in the procedures of comprehensive treatment for degeneration of intervertebral disc.

OBJECTIVE:

To observe the pathological and imaging changes of lumbar intervertebral disc degeneration after Ad/CMV-Sox9 transduction.DESIGN,TIME AND

SETTING:

The randomized controlled experiment based on genes was performed in the Center Laboratory of the First Clinical College,Harbin Medical University between October 2005 and March 2006.MATERIALSAd/CMV-Sox-9 and Ad/CMV-green fluorescent protein were supplied by the Vector Gene Technology Co.,Ltd(Beijing,China).

METHODS:

Thirty New Zealand white rabbits,which had been created lumbar intervertebral disc degeneration models,were divided into 2 groups by the random number table.20 rabbits in the Ad/CMV-Sox9 group were exposed lumbar intervertebral disc,and injected Ad/CMV-Sox-9 20 ?L in each segements of L2-3,L3-4,L4-5 after pricking with 0.1 mL syringe.10 rabbits in the expression of green fluorescent protein group were injected isodose Ad/CMV-GFP.MAIN OUTCOME

MEASURES:

The expression of mRNA collagen II was evaluated at 2,4,8 weeks after transfected with Sox-9.?-actin was served as blank control to observe pathological and imaging changes of lumbar intervertebral disc degeneration.

RESULTS:

The Ad/CMV-Sox-9 was transfected with intervertebral disc degeneration successfully.The expression of mRNA collagen II was obvious higher than that in the blank control group(P