Using recombined SSB antigen expressed in Pichia pastoris to detect anti-SSB antibody by dot immunogold filtration assay / 临床检验杂志
Chinese Journal of Clinical Laboratory Science
;
(12)2006.
Artículo
en Chino
| WPRIM
| ID: wpr-593118
ABSTRACT
Objective To establish a new,rapid,simple and reliable assay for detecting autoantibody SSB.Methods A new dot immunogold filtration assay(DIGFA) was developed,in which the recombinant SSB protein expressed in Pichia pastoris was bound to nitrocellulose(NC) membrane and colloidal gold-labeled staphylococus protein A(SPA) was used as an indicator.Results The sensitivity and specificity of DIGFA were 100% and 98.75%,respectively.The agreement between DIGFA and ENA dot assay was 99.01%.Conclusion DIGFA for detecting autoantibody SSB is a good,rapid,simple and accurate assay for clinical diagnosis.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Idioma:
Chino
Revista:
Chinese Journal of Clinical Laboratory Science
Año:
2006
Tipo del documento:
Artículo
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