Anti-convulsion action of histamine H3 receptor antagonists to rat model with intractable epilepsy / 吉林大学学报(医学版)

ABSTRACT

Objective To explore the anti-convulsion action of histamine(HA) in the central system and treatment of histamine H3 receptor(H3R) antagonists to rat model with intractable epilepsy.Methods 88 Wistar rats(12-day-old) were randomly divided into three groupsnormal control group,N-methyl-D-aspartate(NMDA) group and betahistine(BH) groups(including high and low dose BH groups).Wistar rats received an intraperitoneal NMDA administration to make animal model of intractable epilepsy at infant period and toddler age.After that,the rats were observed daily for latencies and incidences to two NMDA-dependent stereotypical behaviors.The HA content of each brain region was determined with fluorimetry,and H3R were evaluated with immunohistochemical method.Results The automatisms including tail twisting and emprosthotonus seizures of(12-17)-day-old rats were observed in NMDA and BH groups.The rats,aged 18-25 d,became quiet following automatisms rather than emprothotonic.Compared with NMDA group,BH groups had longer latencies and lower incidences of tail twisting and emprosthotonus(P0.05).Conclusion The NMDA-induced model is similar to the clinical manifest of human West syndrome.It is up to animal model of intractable epilepsy at infant period and toddler age.The HA content of brain region is negatively related with seizure incidence.H3R antagonists have certain therapeutic function to intractable epilepsy in rats at infant period and toddler age.