Expression of signal transduction pathway genes in ARDS transcription profiles of rats / 基础医学与临床

ABSTRACT

Objective To study the signal transduction pathway genes in acute respiratory distress syndrome(ARDS) of rats by gene chip. Methods Total RNAs were isolated from lungs of normal and ARDS rats respectively. The RNAs were purified by oligotex. Both mRNAs from two kinds of tissues were reversely transcribed to cDNA with incorporation of fluorescent-labelled dUTP to prepare the hybridization probes. The mixed probes were hybridized to cDNA microarray. Picture signals of fluorescence in gene array were scanned and compared by CapitlBio Molecule Annotation System V4.0. Quantitative fluorescence RT-PCR was used to validate the results of genechip. Results The results showed that there were 2 genes up-regulated and 9 genes down-regulated in lung tissue of ARDS rats,and these genes were involved in 11 signal transduction pathways. Conclusion Many genes of signal transduction pathways expressed in ARDS transcription profiles of rats.