Genotype Distribution in Extended-spectrum ?-lactamases-producing Escherichia coli / 中华医院感染学杂志

ABSTRACT

OBJECTIVE To investigate the genotype distribution in extended-spectrum ?-lactamases-producing Escherichia coli in our hospital.METHODS Clinical strains of E.coli confirmed to produce ESBLs were collected from our hospital.ESBLs genotype was analyzed by plasmid conjugation,PCR and nucleotide sequencing analysis.RESULTS The susceptiblility rate of ESBLs-producing strains were 100% to imipenem,75.4% to piperacillin/tazobactam.A total of 4 genotypes were identified in the 28 ESBLs-producing strains.CTX-M type was identified in 92.9% of the strains,including CTX-M-14,CTX-M-24 and CTX-M-3.SHV Type was identified in 7.1% of the strains,including SHV-12.CONCLUSIONS ESBLs producers are common in E.coli isolated from our hospital.Most of them are multidrug resistant and CTX-M is the main genotype of ESBLs.Other genotypes of ESBLs are not found.