Preventing Transmision of Microorganisms by Bionic Humidifier During Oxygen Humidification:Laboratory Study / 中华医院感染学杂志

ABSTRACT

OBJECTIVE To investigate the transmission of microorganisms by humidifier during oxygen humidification and search an ideal way to prevent it.METHODS Two kinds of humidifier,the unique bionic humidifier and the bubble humidifier,were involved.The humidification material inside the humidifiers were all contaminated with Staphylococcus aureaus,Escherichia coli and Candida albican at 5?105/ml and 2.5?106/ml.Oxygen passed through every contaminated humidifier to the end of oxygen tube which was put in the bottle with 100ml normal saline,lasting 20 minutes,on the 1st,the 2nd,and the 3rd day after the humidifiers being contaminated.The fluid in the bottles,1ml each,were cultured respectively.RESULTS The S.aureaus count of 39.5,52.4,117.3 CFU/ml in 5?105/ml group,83.1,125.2,263.2 CFU/ml in 2.5?106/ml group and E.coli count of 12.9,29.4,37.3 CFU/ml in 5?105/ml group,26.5,55.2,65.2 CFU/ml in 2.5?106/ml group were detected at the end of bubble humidifier on three consecutive day respectively.There were no positive culture of bacteria at the end of bionic humidifier on three consecutive day respectively.The difference of S.aureaus and E.coli count between bubble and bionic humidifier on three consecutive day was significant(P