Effects of human S100A6 on ?-catenin in two human osteosarcoma cell lines / 基础医学与临床

ABSTRACT

Objective To investigate the effects of human S100A6 on ?-catenin in human osteosarcoma cell lines MG63 and U2OS.Methods Cell lines MG63 and U2OS were infected by recombinant adenoviruses carrying human S100A6 and its siRNA gene,AdS100A6 and AdSiS100A6 respectively,to up-regulate and down-regulate the expression of S100A6. Then RT-PCR,Western blot and immunocytochemistry were used to detect mRNA and protein (level and/or distribution) of ?-catenin.Results In both cell lines,with up-regulated S100A6,expression of ?-catenin mRNA and protein increased(P