Effect of m-nisoldipine on the Ca~(2+)/CaM/CaN signal pathway in 5-HT-induced proliferation of rat PASMCs / 药学学报
Acta Pharmaceutica Sinica
;
(12)2010.
Artículo
en Chino
| WPRIM
| ID: wpr-596860
ABSTRACT
This study is to explore the activation of the Ca2+/CaM/CaN signal pathway in 5-HT-induced proliferation of rat pulmonary artery smooth muscle cells (PASMCs) and the inhibitory effect of m-nisoldipine (m-Nis) on this pathway. PASMCs were cultured with the explant technique. The proliferation of PASMCs was evaluated by MTT assay. Confocal microscopy was used to measure the change of [Ca2+]i. The mRNA expression of CaM and CaN was evaluated by RT-PCR and the activity of CaN was measured according to the instruction of kits. The results of MTT assay suggested that 5-HT (1 ?mol?L-1) significantly induced the proliferation of rat PASMCs (P
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Idioma:
Chino
Revista:
Acta Pharmaceutica Sinica
Año:
2010
Tipo del documento:
Artículo
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