Effects of different types of cell culture dishes on fluorescent image with confocal microscope / 国际生物医学工程杂志
International Journal of Biomedical Engineering
;
(6): 177-180,后插5,后插6, 2012.
Artículo
en Chino
| WPRIM
| ID: wpr-598030
ABSTRACT
Objective To investigate the effects of different thickness of cell culture dishes on fluorescent image with confocal microscope.Methods The fluorescent staining experiments of live cells and fixed cells were used to determine the differences among three dishes with different thickness coverslips of 0.085~0.13 mm,0.13~0.16 mm and 0.16~0.19 mm,while the cell appearance,fluorescence lightness and mean of fluorescence intensity were studied with confocal microscope.Results Demonstrated by the results of cytoskeleton staining experiments,the dish with 0.13~0.16 mm thickness coverslip was the best choice for confocal microscope,the dish with 0.16~0.19 mm thickness coverslip was the second one,the dish with 0.16~0.19 mmthickness coverslip was the last one.ConclusionThe dish with 0.13~0.16 mm thickness coverslip is the best choice for confocal microscope.On this type of dish,the cytoskeleton is unfolding and clear after staining.The intensity of fluorescence is the strongest,and the imaging effect is the best.
Texto completo:
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Índice:
WPRIM (Pacífico Occidental)
Idioma:
Chino
Revista:
International Journal of Biomedical Engineering
Año:
2012
Tipo del documento:
Artículo
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