Relation between perfusion speed and acute separation of myocardial cells in New Zealand rabbits / 心血管康复医学杂志
Chinese Journal of cardiovascular Rehabilitation Medicine
;
(6): 40-43, 2013.
Artículo
en Inglés
| WPRIM
| ID: wpr-598151
ABSTRACT
Objective:
To establish a simple, stable and high success rate separation method of myocardial cells in New Zealand rabbits.Methods:
Compared influence between perfusion speed of 50 r/min (8 ml/min) and 65 r/min (11 ml/min) of Langendorff perfusion device on cell separation during separation course of myocardial cells.Results:
Compared with perfusion speed of 50 r/min, there was significant decrease in time to obtain cells [(33±9.10) min vs. (19.29±2.73) min], and significant increase in success rate (50% vs. 91%) and survival rate [(38.26±2.63) % vs. (85.71±2.21) %] by perfusion speed of 65 r/min, P<0.01 all.Conclusion:
Perfusion speed is an important factor that affects acute separation of myocardial cells in New Zealand rabbits, and 65r/min perfusion speed may be more stable and successful to obtain single myocardial cell.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Idioma:
Inglés
Revista:
Chinese Journal of cardiovascular Rehabilitation Medicine
Año:
2013
Tipo del documento:
Artículo
Similares
MEDLINE
...
LILACS
LIS