Effect of PDGFR-αASODN/lip2000 complex implantation into vitreous cavity for proliferative vitreoretinopathy in rabbit / 实用医学杂志

ABSTRACT

Objective To examine the effect of PDGF-αreceptor on proliferative vitreoretinopathy (PVR) in rabbits. Methods Different concentrations of PDGFR-α ASODN were mixed with lip 2000, and the final proportion of PDGFR-α ASODN/lip2000 complex was 1∶1、1∶2.5 and 1∶5 respectively. All the complexes were incubated with cultured human retinal pigment epithelium for 24 hours before transfection. The rabbits were divided into group A (RPE cells)、group B and C (1.0、2.0 μmol/L PDGFR-αASODN lipofectin transfection of RPE cells) with 8 eyes each. The level of PVR were estimated by indirect ophthalmoscope examination; the fundus changes were estimated by histopathology; and the expression of PDGFA was detected by immunohistochemistry. Results The highest transduction efficiency was PDGFR-α ASODN/ lip2000 ratio to 1∶2.5. The degree of proliferative vitreoretinopath , the fundus changes and the density of PDGFA in group B and group C were significantly lower than that in group A, while group C more lower than group B. Conclusion PDGF-αreceptor antisense oligonucleotides can inhibit the development of experimental proliferative vitreoretinopathy.