Determination of Psoralen, Isopsoralen, Icariin and BaohuosideⅠin Shengrong Yanling Pill by HPLC Elution Method / 中国中医药信息杂志

ABSTRACT

Objective To develop an HPLC method for determination of the contents of psoralen, isopsoralen, icariin and baohuosideⅠin Shengrong Yanling Pill. Methods Hypersil C18 column was used as the chromatographic column, with gradient elution, the flow rate of 1.1 mL/min, the mobile phase A of acetonitrile-methanol (2∶1), the mobile phase B of 0.1% glacial acetic acid solution, and detection wavelength of 246 nm (for psoralen and isopsoralen) and 270 nm (for icariin and baohuoside Ⅰ). Results There was a good linear relationship between the injection volume and peak area valued when the volume of psoralen, isopsoralen, icariin and baohuosideⅠwere within the range of 0.033 2-0.664 0 μg (r=0.999 8), 0.022 6-0.452 0 μg (r=0.999 1), 0.029 4-0.588 0 μg (r=0.999 5), and 0.024 2-0.484 0 μg (r=0.999 4), respectively. The average recovery rates were 97.48% (RSD=0.92%), 98.44% (RSD=1.39%), 99.13% (RSD=1.17%) and 98.71% (RSD=1.21%), respectively. Conclusion The method was convenient, accurate, sensitive and repeatable, and could be used in the content control method of psoralen, isopsoralen, icariin and baohuosideⅠin Shengrong Yanling Pill.