Simultaneous determination of seven active ingredients in Xiongju Shangqing pills by HPLC / 中国生化药物杂志

ABSTRACT

Objective To establish a method for simultaneous determination of seven active ingredients(Jasminoidin,Forsythoside A,Caffeotannic acid,3,5-Di-O-caffeoylquinic acid,Jatrorrhizine Hydrochloride,Baicalin,Berberine Hydrochloride).Methods The determination was carried out with Agilent Poroshell 120 SB-C18(100 mm ×3.0 mm,2.7 μm);The mobile phase consisted of acetonitrile(A)-0.1% phosphoric acid solution(B)with gradient elution at a flow rate of 1.0 mL/min;The detection wavelengths were 230 nm in 0~4.5 min(determination of Jasminoidin),348 nm in 4.5 ~9.0 min(determination of Forsythoside A,Caffeotannic acid,3,5-Di-O-caffeoylquinic acid),and 278 nm in 9.0 ~15.0 min (determination of Jatrorrhizine Hydrochloride;Baicalin;Berberine Hydrochloride).Results The calibration curves were linear in the ranges of 4.024~80.48μg/mL for Jasminoidin(r=0.9991),2.042~40.84μg/mL for Forsythoside A(r=0.9993),3.956 ~79.12 μg/mL for Caffeotannic acid(r=0.9996),6.090 ~121.80μg/mL for 3,5-Di-O-caffeoylquinic acid(r=0.9990),4.026 ~80.52 μg/mL for Jatrorrhizine Hydrochloride(r=0.9995),3.924~78.48 μg/mL for Baicalin(r=0.9998),6.030~120.6μg/mL for Berberine Hydrochloride(r=0.9998).The average recoveries(n=6)were 96.5%,97.5%, 95.9%,98.6%,99.8%,98.4% and 97.3% ;RSDs were 1.5%,1.3%,1.2%,1.4%,1.2%,1.1% and 0.9% respectively.Conclusion The method is simple and repeatable,which can be applied to the quality control of Xiongju Shangqing pills.