Comparison of BRAF mutation detection in patients with papillary thyroid microcarcinoma by ARMS and direct sequencing / 中国免疫学杂志
Chinese Journal of Immunology
;
(12): 1514-1516,1522, 2014.
Artículo
en Chino
| WPRIM
| ID: wpr-600301
ABSTRACT
Objective:
To investigate the sensitivity and the specificity of scorpions amplification refractory mutation system ( ARMS) in comparing with that of direct DNA sequencing in the detection of BRAF gene mutations in patients with papillary thyroid microcarcinoma.Methods:
Direct sequencing and ARMS were used simultaneously to detect BRAF mutation status in 56 patients with PTMC.Results:
BRAF mutations were identified in 46 cases with a mutation rate of 82.9%by ARMS,while in 18 cases with a mutation rate of 32.1%by direct sequencing.Besides,the sensitivity of ARMS was 100%and that of direct sequencing was 39.1%.There were significant differences of both mutation rate and sensitivity between two methods ( P<0.01 ).Conclusion:
Compared to direct sequencing,ARMS gains a higher sensitivity in the detection of BRAF mutations in samples with tiny lesions.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Tipo de estudio:
Estudio diagnóstico
/
Estudio pronóstico
Idioma:
Chino
Revista:
Chinese Journal of Immunology
Año:
2014
Tipo del documento:
Artículo
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