Relationship between transferrin levels and function of male fertility and testicular podocyte / 国际检验医学杂志

ABSTRACT

Objective To investigate the relationship between levels of transferring(Tf)and function of male fertility and testic-ular podocyte.Methods Semen samples from male patients with infertility and volunteers with normal infertility were collected and tested for sperm density and motility by using sperm quality analyzer.In additon to that,Tf concentrations of Tf were determined;aseptically excised rat testis and by collagenase and hyaluronidase digestion,podocytes were isolated with high purity and cultured in culture medium,then the concentration of Tf in cell culture medium was determined;Tf concentration were determined by rate nephelometry.Results Tf concentration in semen of male-infertility patients[(15±5)μmol/L]was significantly lower than that of normal-fertility volunteers[(24.5±6.5)μmol/L,P <0.01],which were positively correlated sperm count and motility(P <0.01). Tf concentrations of cell culture medium[(25 ±8)μmol/L]in which testicular podocytes were cultured were significantly higher than that of infertile rat[(15 ±6)μmol/L,P <0.01].Conclusion Determination of semen Tf levels can reflect podocyte function and be used as an indicator for the evaluation of seminiferous tubules spermatogenesis,which was valuable in the diagnosis and treatment of male infertility.