Detection of Enterobacter cloacae produced AmpC enzymes and ESBLs and study of its multi-drug resistance mechanism / 国际检验医学杂志

ABSTRACT

Objective To explore the prevalence and antimicrobial resistance mechanism of multiple drug resistance Enterobacter cloacae in ShenZhen region .Methods We screened 72 strains of multi resistant strains from 82 strains of Enterobacter cloacae iso‐lated from on clinical .And detected extended spectrum beta lactamases (ESBLs) and sustained high AmpC enzyme with ESBLs phenotypic confirmatory test and flucloxacillin (FCC ) double inhibitor diffusion synergy test method (DIDST ) .Results In 82 strains of Enterobacter cloacae isolated on clinical ,33 strains (40 .2% ) derepressed Hyperproducing AmpC enzymes ,25 strains (30 .5% ) produced ESBLs ,14 strains (17 .1% ) produced AmpC enzyme and ESBLs parallelly ,10 strains (12 .2% ) didn′t express the two enzymes .Conclusion High expression of AmpC enzyme and ESBLs is the main mechanism of cephalosporin resistant En‐terobacter cloacae .High AmpC enzyme and ESBLs production and widely used of beta amide antibacterial drug have a direct rela‐tionship .The first choice of treatment of multi drug resistant Enterobacter cloacae should be carbapenem .