Neuroprotective Effect of Ginkgolides on Cultured Dorsal Root Ganglion Neurons with Neurotoxicity Induced by Glutamate / 天津医药

ABSTRACT

Objective:To determine the neuroprotective effect of ginkgolides(Gin)on cultured rat embryos dorsal root ganglion(DRG)neurons injured by glutamate(Glu)in vitro.Methods:DRG neurons of Wistar rat embryos were cultured in vitro for 48 h and then exposed to Glu(200 μmol/L)with or without Gin(50 μmol/L).The living cells were observed with an inverted contrast microscope.The cultures were processed for detecting the apoptosis rate by using flow cytometry.The fluorescent intensity of intracellular Ca2+ was detected by confocal laser scanning microscope(CLSM).Results:The living status of DRG cells with Gin incubation was better than that of incubated cultures without Gin.The shape of neuronal cell bodies or neurite networks were almost the same in the glutamic acid group with Gin compared with that of the normal control group.The apoptosis rate of cells incubated with Glu for 24 h was 41.1% in DRG cultures.The apoptosis rate of cells incubated with Glu and 50 μmol/L of Gin for 24 h was 7.6% in DRG cultures.The fluorescent intensity was lower in Gin with Glu group than that in Glu group(P＜0.01).The fluorescent intensity was lower in the control group than that in Glu group(P＜0.01).There was no significant difference in the fluorescent intensity between Gin with Glu group and control group(P＞0.05).Conclusion:Ginkgolides may reduce intracellular Ca2+ concentration,and then protect DRG neurons from neurotoxicity induced by Glu.