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Genetic Alterations in Intrahepatic Cholangiocarcinoma as revealed by Degenerate Oligonucleotide Primed PCR-Comparative Genomic Hybridization
Journal of Korean Medical Science ; : 682-687, 2004.
Artículo en Inglés | WPRIM | ID: wpr-60323
ABSTRACT
Intrahepatic cholangiocarcinoma (ICC), a malignant neoplasm of the biliary epithelium, is usually fatal because of difficulty in early diagnosis and lack of availability of effective therapy. The genetic mechanisms involved in the development of ICC are not well understood and only a few cytogenetic studies of ICC have been published. Recently, technique of degenerate oligonucleotide primed (DOP)-PCR comparative genomic hybridization (CGH) permits genetic imbalances screening of the entire genome using only small amounts of tumor DNA. In this study chromosomal aberrations in 33 Korean ICC were investigated by DOP-PCR CGH. The common sites of copy number increases were 20q (67%), 17 (61%), 11q11-q13 (42%), 8p12-qter (39%), 18p (39%), 15q22-qter (36%), 16p (36%), 6p21 (30%), 3q25-qter (27%), 1q41-qter (24%), and 5p14-q11.2 (24%). DNA amplification was identified in 16 carcinomas (48%). The frequent sites of amplification were 20q, 17p, 17q23-qter, and 7p. The most frequent sites of copy number decreases were 1p32-pter (21%) and 4q (21%). The recurrent chromosomal aberrations identified in this study provide candidate regions involved in the tumorigenesis and progression of ICC.
Asunto(s)

Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Oligonucleótidos / Neoplasias de los Conductos Biliares / Conductos Biliares Intrahepáticos / Reacción en Cadena de la Polimerasa / Aberraciones Cromosómicas / Cartilla de ADN / Colangiocarcinoma / Dosificación de Gen / Hibridación de Ácido Nucleico Tipo de estudio: Estudio de tamizaje Límite: Adulto / Anciano / Femenino / Humanos / Masculino Idioma: Inglés Revista: Journal of Korean Medical Science Año: 2004 Tipo del documento: Artículo

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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Oligonucleótidos / Neoplasias de los Conductos Biliares / Conductos Biliares Intrahepáticos / Reacción en Cadena de la Polimerasa / Aberraciones Cromosómicas / Cartilla de ADN / Colangiocarcinoma / Dosificación de Gen / Hibridación de Ácido Nucleico Tipo de estudio: Estudio de tamizaje Límite: Adulto / Anciano / Femenino / Humanos / Masculino Idioma: Inglés Revista: Journal of Korean Medical Science Año: 2004 Tipo del documento: Artículo