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Effects of Cytokine Milieu Secreted by BCG-treated Dendritic Cells on Allergen-Specific Th Immune Response
Journal of Korean Medical Science ; : 640-646, 2004.
Artículo en Inglés | WPRIM | ID: wpr-60331
ABSTRACT
Bacillus Calmette-Guerin (BCG) is reported to suppress Th2 response and asthmatic reaction. Dendritic cells (DCs), the major antigen-presenting cells, infections with BCG are known to result in inducing various cytokines. Thus, DCs are likely to play a role in the effects of BCG on asthma. This study aims at investigating that cytokine milieu secreted by BCG-treated DCs directly enhances allergen-specific Th1 response and/or suppresses Th2 response in allergic asthma. DCs and CD3+ T cells were generated from Dermatophagoides farinae-sensitive asthmatics. DCs were cultured with and without BCG and subjected to flow cytometric analysis. IL-12 and IL-10 were determined from the culture supernatants. Some DCs were cocultured with T cells in the presence of D. farinae extracts after adding the culture supernatants from BCG-treated DCs, and IL-5 and IFN-gamma were determined. BCG-treated DCs enhanced significantly the expressions of CD80, CD86, and CD40, and the productions of IL-12 and IL-10. Addition of culture supernatants from BCG-treated DCs up-regulated production of IFN-gamma by T cells stimulated by DCs and D. farinae extracts (p0.05). The cytokine milieu secreted by BCG-treated DCs directly enhanced allergen-specific Th1 response, although did not suppress Th2 response.
Asunto(s)

Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Asma / Células Dendríticas / Activación de Linfocitos / Regulación hacia Arriba / Células Cultivadas / Citocinas / Interleucina-5 / Interferón gamma / Interleucina-10 / Células Th2 Límite: Humanos Idioma: Inglés Revista: Journal of Korean Medical Science Año: 2004 Tipo del documento: Artículo

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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Asma / Células Dendríticas / Activación de Linfocitos / Regulación hacia Arriba / Células Cultivadas / Citocinas / Interleucina-5 / Interferón gamma / Interleucina-10 / Células Th2 Límite: Humanos Idioma: Inglés Revista: Journal of Korean Medical Science Año: 2004 Tipo del documento: Artículo