Primary culture of rabbit fibroblast-like synoviocytes using an improved explant culture method / 中国比较医学杂志
Chinese Journal of Comparative Medicine
;
(6): 75-78,85, 2016.
Artículo
en Chino
| WPRIM
| ID: wpr-603373
ABSTRACT
Objective To establish an easier and better method for primary culture of rabbit fibroblast-like synoviocytes(FLS) by modified tissue cell culture.Method Healthy rabbit joint synovial layers were cut into small pieces and siphoned off water attached on the tissue with sterile filter paper and then cultured.The growth status and morphology were observed.The growth curve of the 3rd passage cells was measured by CCK-8 assay, and the expression of vimentin was detected by immunocytochemistry.Results The FLS cultured in vitro exhibited a spindle-shaped appearance and could rapidly expand.The cell growth curve was typical of S type, and the cells highly expressed vimentin.Conclusions Primary culture of rabbit FLS by the improved explant culture method is successfully established.The method is simple and highly efficient.It provideds a new method for isolation of FLS.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Idioma:
Chino
Revista:
Chinese Journal of Comparative Medicine
Año:
2016
Tipo del documento:
Artículo
Similares
MEDLINE
...
LILACS
LIS