Discussed the influence of the stability that laboratory repeated use the HIV quality control serum during the cold storage / 国际检验医学杂志

ABSTRACT

Objective To investigate the effect of repeated use on the stability of HIV quality control serum during cold storage , and put forward measures to deal with .Methods Ten HIV serum from -20 degrees celsius freezer room temperature balance out , 30 minutes to make it completely melted ,randomly arranged in the patient sample ,added sample testing according to the normal sample ,after the end of time to save 2 to 8 degrees celsius refrigerator .The next day ,the above quality control products from 2 to 8 degrees celsius refrigerator ,after 30 minutes at room temperature ,randomly arranged in the patient sample ,add sample testing ac-cording to the normal sample ,after the end of time to save 2 to 8 degrees celsius refrigerator ,repeat the above activities to seventh days .Using repeated measure ,at the same time ,took the average 10 quality control of product values on the same day as a check-pointcompared with daily quality control chart ,observe changes .Results It suggested that in different measurement on the num-ber of days the difference was statistically significant(F=48 .515 ,P<0 .01) ,combined with the quality control chart found ,which was starting from the fourth day quality value warningstatus ,began to appear and the drift of fell out of control .Conclusion HIV quality-control serum at 2 to 8 degrees celsius refrigerator ,used repeatedly during 3 days late stability is affected ,the change of quality control diagram appear drift ,even is out of control .