Determination of Emodin and Danshensu in Pinggan Oral Liquid by HPLC / 中国药师
China Pharmacist
;
(12): 758-760,761, 2016.
Artículo
en Chino
| WPRIM
| ID: wpr-603816
ABSTRACT
Objective:
To establish the method for the determination of emodin and danshensu Pinggan oral liquid.Methods:
The contents were determined by HPLC. The chromatographic conditions for emodin were as follows an Agilent Eclipse XDB-C18 column (150 mm × 4. 6 mm, 5μm) was used and the mobile phase was methanol-water (80 ∶20). The detection wavelength was 287 nm and the flow rate was 1. 0 ml·min-1 . The column temperature was 25℃ and the injection volume was 20 μl. The chromatographic condi-tions for Danshensu were as followsan Agilent Eclipse SB-C18 column (150 mm × 4. 6 mm, 5 μm) was used and the mobile phase was methanol-1% acetic acid solution (2 ∶98). The detection wavelength was 280 nm and the flow was 1. 0 ml·min-1. The column temperature was 30℃ and the injection volume was 20 μl.Results:
Emodin had a good linear relationship within the range of 26. 75-428. 00 μg·ml-1(r=0. 999 9). The average recovery was 102. 7% and RSD was 0. 76% (n=6). Sodium Danshensu had a good linear relationship within the range of 10.17-162.77 μg·ml-1(r =0.999 9). The average recovery was 100.5% and RSD was 1. 47% (n=6).Conclusion:
The method is simple, accurate and reproducible, which can be used for the quality control of the prep-aration.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Idioma:
Chino
Revista:
China Pharmacist
Año:
2016
Tipo del documento:
Artículo
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