Improvement of Quality Standard for Shenyan Ⅱ Granules / 中国药师
China Pharmacist
;
(12): 1008-1010,1011, 2016.
Artículo
en Chino
| WPRIM
| ID: wpr-604253
ABSTRACT
Objective:
To establish the quality standard for Shenyan Ⅱ granules.Methods:
The characteristics of Folium Pyrrosiae were identified by microscopy. The three herbs in the preparation,rehmannia,madder and thistle were identified by TLC qualitatively. The content of ginsenoside in American ginseng was determined by HPLC. Chromatographic separation was carried out by using a WONDER CRACT ODS-2(150 mm × 46 mm,5 μm)column. The mobile phase consisted of acetonitrile-0. 1% phosphoric acid(28 ∶72)with gradient elution at a flow rate of 1. 0 ml·min -1 ,and the injection volume was 10 μl. The detection wavelength and the column temperature was 203 nm and 40 ℃,respectively.Results:
The spots in TLC were clear without any interference. The linear range of ginsenoside was 38. 9- 194. 5 μg·ml -1(r = 0. 999 3). The average recovery was 98. 3% and RSD was 1. 9%(n = 6).Conclusion:
The method is simple and highly specific with good repeatability,which can be used as the quality control method for Shenyan Ⅱ granules.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Tipo de estudio:
Estudio pronóstico
Idioma:
Chino
Revista:
China Pharmacist
Año:
2016
Tipo del documento:
Artículo
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