Raman Tweezers Techniques in Different Cells Research / 中国医学物理学杂志

ABSTRACT

Objective: To introduce the Raman spectrum, the optical tweezers physical principle and its working characteristics, unify the detail medical research work, summarize specific application situation of the Raman tweezers technology in the different cells domain. Methods: Use the optical tweezers to fix the living cells, simultaneously carry out Raman spectrometry on the living cell or the cell organ by using the laser Raman technology. By applying this technology, the samples will be captured in the suspending liquid. In an approximate physiological state, the single living specimens, such as the cells, the cell organs or the biological macro-molecules, will be studied and the real-time track to the research object physiological biochemistry process will be carried on, then the Raman spectrometry will be implemented to the living cells. Results: From the single cell level, Raman tweezers technology analyzes the oxygen ability and the deformability of red blood cells of normal persons and the Mediterranean Sea anemia patients, and implements the appraisal of blood red cell and the blood platelet of different species. The Raman tweezers technology reveals the differences between the organizational structure of the cancer cells and that of normal cells in the molecular level, providing important information and data for the cancer diagnosis and the mechanism analysis. The Raman tweezers technology has implemented the torsion and knotting of DNA molecules, and realizes the control and differentiation of human being's chromosome. Conclusion: The Raman tweezers technology is the prompt and effective tool for the real-time research of cell physiology and biochemistry changes, hopefully in the molecular level. It will become one of the most advanced tools to carry out examination and diagnosis of different kind of living cell. Surely it has a very bright prospect.