Determination of the Main Content and Entrapment Efficiency in the Brucine Nanostructured Lipid Carrier by HPLC / 中国药房
China Pharmacy
;
(12): 2983-2985,2986, 2015.
Artículo
en Chino
| WPRIM
| ID: wpr-605084
ABSTRACT
OBJECTIVE:
To establish a method for determining the main contents and entrapment efficiency in the bruc-ine nanostructured lipid carrier(NLC).METHODS:
HPLC was adopted to determine the main content,sephadex gel filtration meth-od was employed to separate free drug in brucine NLC to determine the entrapment efficiency. The column was Dikma C18 with the mobile phase of mobile phase A(methanol)-mobile phase B [water-acetic acid-triethylamine(230∶2.4∶0.3,V/V/V)](30∶70,V/V)at the flow rate of 1 ml/min,the detection wavelength was 265 nm,volume was 10 μl and temperature was 30 ℃.RESULTS:
The linear range of brucine was 4.00-80.00μg/ml(r=0.999 9);RSDs of precision,stability and reproducibility tests were≤1.67%;av-erage recoveries of content determination and sephadex gel filtration method were respectively 99.66%(RSD=0.45%,n=9) and 99.75%(RSD=1.74%,n=9);and the average entrapment efficiency was 69.92%.CONCLUSIONS:
The method is simple,re-producible and efficient,and can be used for the determination of main contents and entrapment efficiency in brucine NLC.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Idioma:
Chino
Revista:
China Pharmacy
Año:
2015
Tipo del documento:
Artículo
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